Monday, March 30, 2009

Ep 4- Taster: stem cells

As a taster of what will be on in tomorrow nights episode of the Petridish at 10pm on Joy 94.9FM here is the annotation of one of the segments, Science News. Also if you are a twitter user, insert #pdish into your message and we can track it, and follow through summize


Reversing Birth Defects

During neural development, when there are prenatal teratogon (substances which cause neural damage) act diffusely through the brain. The University of Jerusalem [30.12.08] in mice, by inserting stem cells into the brain, were able to overcome damage as the stem cells migrated to the site of the damage tissue and differentiated to repair the region.

The mice who were exposed to pesticides and heroin while pregnant offspring are generally heavily retarded, but once treated with direct neural stem cell transplantation into the neural stem behavioural tests showed normality as well the brain chemistry recovering to normal.

This brings us closer to overcoming brain damage done during maturation can be reversed, giving hope to parents who may have been exposed to teragons during pregnancy.


Not Just a Product

Research at the University of California [28.1.09] has found that they could program pluripotent stem cells into precursors of the germ-line cells that produce sperms and eggs. This could answer issues of infertility caused by injury or disease.

Reprogrammed Skin cells into induced pluripotent stem cells (iPS) now give a method for the production of germ-line cells, but there are major differences between those of iPS and Human embryonic Stem cells (HES), which means that further investigation is needed to determine what the differences mean in the way of fertility, and functionality of the resultant gametes. Because of this further research is necessary before the new found results are to be implemented.


Not All a Good Thing

According to Wiley-Blackwell [5.11.08] published in Clinical and Transitional Science:

“Breast and other cancers are maintained through a population of cancerous stem cells.”

This statement came out in relation to the over expression of cyclin d1 being an over expressed protein in stem cell based cancers such as nodule breast cancer. The role that has been found for this is now giving way to better treatment and understanding “By specifically targeting…[them] we hope to reduce the reoccurrence and improve therapy” says Pestell.

Lindsay et al ErbB2 Induces Notch 1 Activity and Function in Breast Cancer. Clinical and Translational Science, 2008;1 (2):107D01: 10.1111/j.1752-8062.2008.00041.x

Stem Cells Used in Cure for Type 1 Diabetes

Published in Developmental Cell, Baylor College of Medicine [17.3.2009] have found a gene which can induce insulin production.

Neurogenin3 is delivered by a disarmed virus (which attacks the liver) causes blood sugar to plummet to their correct level, but this doesn’t work with mature liver cells, but with the stem cells around the portal vein (called islet cells) and trigger them to start producing insulin.

The virus used in the study on mice may however be harmful to people, and so more refinement is needed before human testing.

Sunday, March 22, 2009

Episode 3: Cookies and Addiction

How we Get Addicted

A reward circuit with a stress response primarily causes addiction, which is in part is due to a connection between the Amygdala and the Nucleus Accumbins.

The Amygdala is apart of the limbic system, which is situated in the brain near the blood supply, and so due to its proximity to the blood supply, it is very sensitive to changes in blood sugars, and other changes, and is used for emotional learning. The Nucleus Accumbins sits in the frontal lobe, and has an important role when it comes to fear, laughter, pleasure and placebo responses.

So the Nucleus accumbins alters your mood in response to thought of stimulus, and the Amygdala is directly impacted by stimuli.

The two sections are very closely associated,, the main function of the 2 of these is to get us to learn what is good and bad for survival. [explanation:] so sweet cookies give us sugar, the amygdala records that the sugar has increased, the nuclease accumbins, knows that we had a cookie, so if we go out and get more cookies, we wil get more sugar spikes.

The neurotransmitter that is associated with this is DOPAmine, so things that can cause an increase in a DOPAminergic response (fancy word) can trigger a positive association with the substance. So Heroin, which acts like Dopamine, or Cocaine, which blocks its re-uptake increase the response, giving positive re-enforcement, can create a neural cycle of addiction. the problem with this is that in the Amygdala, dopamine is also used for a pleasure response, and when there is a lot of something, your body tries to even itself out, so it tries to achieve homeostasis.

So your Brain tries to make itself less sensitive to dopamine, and so the threshold response is decreased, so the amount needed to get the same kind of hit is more than previous time.

Also the Brain can stop producing dopamine, which is also important in things like controlling inhibitory movements, and so can cause Parkinson disease if there is isn't enough.

Also chemicals can be released to that block dopamine from binding as a neurotransmitter, this can cause permanent depression.

Barbiturates also induce the release of glutamate's in high concentrations that starts to kill off the neurons, which should be causing the overproduction, but glutamate's are in-discriminate, and also take out other surrounding neurons, so it can cause a myriad of neural disorders.

It doesn't necessarily take multiple goes to cause this; a single hit can cause addiction

New information on Addiction

There are also genetic factors that attribute to addictions,

According to the April 2009 Nature Review Genetics, the university of Virginia and the University of Michigan found several genes associated with multiple addictions.

Now there are several genes already been identified with addiction, such as modifications of the:

GABRA2

ANKK1

Neurotoxin 1 & Neurotoxin 2

But 3 new ones have been found, and there is an overlapping in different addiction types to different genetic markers such as:

CHRNA 5

CHRNA 3

CHRNB4

ssociations made between them, now with a mapping of where these addictions are, means personalized treatments can now be constructed to ease treatments. But the reasons for why these markers cause the addictions are still unknown.

Can the Cookie Monster Become Addicted to Cookies??

So sweet cookies give us sugar, the amygdala records that the sugar has increased, the nuclease accumbins, knows that we had a cookie, so if we go out and get more cookies, we will get more sugar spikes.

So the cookie monsters compulsive eating of the cookies means that sugar rush that the cookie monster got from his 1 cookie is now not nearly as effective as giving him his sugar rush (kinda like your first cup of coffee), and so he needs to eat more and more cookies. Each time with the positive sugar reinforcement and the insulin hit. And when he doesn't get a cookie, he seems down, and when there are cookies directly in front of him, like the age demographic who he is representing, 3-6 years of age, he doesn't contemplate the guilt of how much time he will have to go to the gym, to burn of the calories that he will be getting from the cookies, as opposed to the potassium and thiamine that he would be getting from a banana.

The problem with his unhealthy cookie addiction is for starters the crazy sugar in insulin spikes that he would be having, so insulin receptors after getting knocked around for so long, (40 years of compulsive cookie cravings) can induce type 2 diabetes.

Because the Brain is kept at a constant sugar level, the way in which the brain recognizes the change in sugar levils in the blood is through an increase in insulin, the hormone released by the liver to induce glucose uptake when levels increase. Because of this, hits of insulin are what give you sugar cravings, which glucagons release, giving the alternative stress response. This can result in a sugar addiction, and whith the stong association with coockies, can result in a cookie addiciton

Now that he is the healthy eating monster, he is eating wholegrain breads and broccoli, which are low GI foods, which when he eats them he gets a small sugar hit, but not as big as when he eats a cookie, but the way that the sensors for blood sugar levels work, because they based in homeostasis, unlike direct dopaminergic responses, he is able to lower the amount of sugar that he needs to get this crazy rush, and so overcome his sugar addiction 1 day at a time.

Saturday, March 21, 2009

Episode 3: Science News

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Episode 2: Carby Fat Fat

This week on the Petri Dish:

Science News (at 1:57 on the podcast)

Science Controversy: Are carbs the enemy? (6:00)

Science Myth: Vegetarianism is bad for you (14:37)

Remember This Fact?: How do we taste? (22:39)

Film of the Week: The Nutty Professor (26:25)


Carbs vs Fats

Fats and carbs are both digestible for energy, which is because they are long chains of carbon molecules and hydrogen along them, which, when digested become carbon dioxide, the same stuff cars make. but the 2 different shapes have different properties. Fats are the simplest as they are 2 long chains like legs negatively charged, and so group together, into sheets, and congregate like crowds.

The most basic of these are the saturated fats, which are like people standing arms and legs held together, meaning that they can all stand closer together, meaning that if your trying to walk through the crowd its harder to push through, and because their all pushed so close together they make a thicker layer as they push up, and so its for transferal from thing from the floor to the surface.

This is why there is such a push against saturated fats, because this rigidity, and inability to allow for molecules to move from the bottom to the top OR VICE VERSA. Due to this too many saturated fats, can lead to weakening of the heart due to having to force against this inflexibility, and puts pressure on the neurons in the brain because of a decrease in plasticity.

Unsaturated fats are like saturated ones but there is a kink in the legs, so its like they’re standing with their legs out. Which puts more space between each of the people. And so it would be easier to walk between them as there is the extra space between them, allowing for better flow through them, and if you dropped your phone you would be able to pick it up and hold it to your head. this extra flexibility adds plasticity.

The OMEGA3 and OMEGA6 oils are fats which have these kinks at the 3rd and sixth Carbon, which is why they are good for your heart, and there was such a fuss over them. they decrease pressure on your heart, allowing for easier beats, and when trying to form new memories, the new connections are easier to produce.

The new "evil fat", the trans fats are called so because they are transition fats, its because they have a load of kinks in them, and because of this they can fold up into cholesterol. what these do in pretty much fold up and connect in the middle making a ring. when they are in the crowd of fats, they are like a short fat person pushing around, mixing up the crowd faster, and so increases the flow of the crowd as they step around, and can even make the saturated fats act like unsaturated fats, with increased plasticity. but when they get together, because they don't have the differing polar head, they congregate, and even if they are flowing past, in the blood, can attach from the outside of the cell. causing arteriole plaques. these trigger immune reactions of white blood cells attaching to remove them, giving addition to the plaques. these can build up until stop the flow of blood which is why cholesterol can cause heart attacks.

Carbohydrates are also used for energy storage, but they are much shorter in their length, only 6Carbons around instead of 12 to 14 like fats, but because they are round and have OH attached to the Carbons they have an easier to attain energy supply and harder to store energy supply. so they are used preferentially to fats. Single carbohydrates are sugars that taste sweet because they are a quick release of energy, and starchy tasting foods are because there are rows of the carbohydrate molecules. These are for storage of them over long periods of times, and so give them a low G.I., which is a glutamic index. This means that the energy released from these are over a long period of time, so there isn't an instant energy hit, which wears off making you hungrier, but its released over a long period of time.

The carbohydrates are placed in strait lines in people, but in vegetables, leaves and grass, the plants have these carbohydrates compacted even more, by branching off all over the chain into a matrix. Our digestive enzymes aren't able to break these complex molecules down in our digestive systems. This is where dietary fiber comes from, and the more of this we eat the faster our bowl movements. And if we eat a high enough diet we can speed out bowl movements up to 12hours from the usual 26-32hours in Europeans.

Hi Carb Low Carb Diets

What is a High Carb Low carb diet?

A high carb diet is where protein and fat intake is decreased, and so their is a minimalism in meats and an increase in food and high in carbohydrates, so generally white things like rice, bread, pasta.

Low protein diets, have a decrease in rice bread and pasta, and so foods high in fibre, protein and fat replace it. so generally meat and roughage in these sorts of diet. Also means or peas, which are generally, low in carbohydrates.

Both the high carb diets and the low carb diets are used as weight loss techniques, and it is because your body burns carbs first, and then fats, then proteins. So if you increase your carbohydrate input and lower your fat input, your body doesn’t have the fats for storage, and to form them has to burn up carbohydrates to convert them.

In a low carb diet, there isn’t the carbs to burn, so fat gets burnt first and then protein. Due to the different digestive properties of these they have differing effects on the body which means that the effect on the body is to decrease the energy input.

The study

In the this Australian study published in the British journal of nutrition they studied both hi and low carb diets in obese people, and observed which is healthier. So specialized diets where set up for the 2 groups and they were monitored over 8 weeks.

the major difference was there was a major decrease in the number of bowl movements (times gone to the bathroom), the mass excreted, and the concentration of butyrate. also there was a decrease in bifidobacteria. This is because this is a bacteria which eats the extra carbohydrates left over in the bowl. These as assumed increased hi-carb diet, but the weird stuff is the things that were the same for both the hi-carb and low carb diet.

The faecal form (colour and shape) pH, ammonia concentration and E.coli did not change. the reason that the faecal form is important is because it displays the health of the intestinal tract, and it was thought that the stool would get darker and greasier in the LC diet. Also when proteins are broken down ammonia is released, but this wasn’t being excreted through the faecal matter, as well as there being no increase in pH, showing that homeostasis keeps most of this under control.

there was no difference between incidence of adverse gastrointestinal symptoms, but with the LC diet there was deteriation in the concentration and excretion of faecal SFCA which is important for immunity cells of the GI tract, in fighting colorectal cancers, also a lowered stool weight, suggesting that there could be long term issues with a LC diet.

Overall the LC diet had faster weight loss compared to the HC diet, but for the long-term the LC diet can be detrimental. part of this is due to the low fibre content of the LC diet. There were no reported such as abdominal pain, or constipation, bloating or excessive flatulence. The decrease in bowl movement time in both diet means that there is an increase in the chance of bowl cancer, because slowed bowl movements means that cells in your bowl have more exposure to chemicals.

Gut transit is not effected by diet.

Reduced memory?

One week after starting a weight loss diet that severely restricted carbohydrates, participants in the Tufts University study performed significantly worse on memory tests than participants who followed a low calorie, high-carbohydrate diet. The low-carb dieters' memory-test performances improved in the following weeks after they began eating some carbohydrates.

The study: 19 women between the ages of 22 to 55 who were closely followed after beginning a low-carb weight loss plan similar to the Atkins diet includes plenty of fruits, vegetables, and whole grains.

Before starting the diets, the women underwent testing designed to measure long- and short-term memory and attention. The tests were repeated one, two, and three weeks after the diet began.

The study is published in the February 2009 issue of the journal Appetite.

Increased memory?

In a study published in 2007, Brinkworth and colleagues performed cognitive function testing on dieters after they had been on either a low-carb or high-carb weight-loss diet for eight weeks. Both groups lost weight and showed improvements in mood.

The low-carbohydrate dieters showed slight impairments in cognitive processing speed, but no difference was recorded between the two groups in working memory. Brinkworth says if eliminating carbohydrates from the diet does affect memory, the effect may only be temporary.

Why would this happen? Carbs Are Brain Fuel

The body breaks carbohydrates into glucose, which it uses to fuel brain activity.

Proteins break down into glycogen, which can also be used for fuel by the brain, but not as efficiently as glucose.

Episode 2: Science News

US President Barack Obama has lifted restrictions on federal funding for research on new stem cell lines. Mr Obama signed an executive order pledging to "vigorously support" new research. Ex-President George W Bush prevented using government money to fund research on human embryonic stem cell lines created after 9 August 2001. "At this moment the full promise of stem cell research remains unknown and it should not be overstated. But scientists believe these tiny cells may have the potential to help us understand and possibly cure some of our most devastating diseases and conditions."

Stem cell solutions:

A procedure that increases a stem cells’ ability to regenerate damaged tissue has been created by the University of New South Wales (UNSW). This procedure could offer new hope to sufferers of muscle-wasting diseases such as muscular dystrophy. Adult stem cells are given a gene making them resistant to chemotherapy. This means that the chemo will kill of the damaged cells, and allow the new stem cells to take hold. The procedure has been successfully used to regrow muscles in a mouse model, but it could easily be applied to all tissue-based illnesses in humans such as in the liver, pancreas or brain.

Endangered Sperm

Researchers at The University of Western Australia compared the sperm of endangered and non-threatened mammals. They analysed sperm data from 20 species including, the panther, lion, cheetah, giant panda, bison and howler monkey. They found that the most inbred species had more sperm abnormalities and fewer mobile sperm, plus inbreeding can severely reduce male reproductive fitness. Endangered species with the most severe loss of genetic variability had the poorest ejaculate quality.

http://www.news.uwa.edu.au/20090304950/business-briefing/sperm-quality-vital-creatures-great-and-small

Prawns do it

Life sciences researcher Gay Marsden, from Queensland University of Technology, spent two months filming prawns making love. She was comparing prawns caught in the wild to prawns reared in captivity. She found that when prawns are caught from the wild and put into tanks, they have no problem breeding. But prawns bred in captivity don’t breed so good. She used infrared cameras, like those used on Big Brother, and compared the sexual behaviour of captive-bred prawns with wild prawns. Her research suggests that the men and women aren’t so keen to do the dirty because the women aren’t releasing the pheromones they need to.

http://www.news.qut.edu.au/cgi-bin/WebObjects/News.woa/wa/goNewsPage?newsEventID=24855

Episode 1: We go ape for evolution

This week on the Petri Dish:

Science News (at 1:49 on the podcast)

Science Myth: Are Dinosaurs Dead? (5:39)

Science Controversy: Can all causcasians really be descended from seven woman? Is the mitochondrial Eve theory true? (9:00)

Remember This Scientific Fact?: Who was Lucy? (15:17)

Film of the Week: Evolution (19:05)


Out of the Trees

Humans and chimpanzees became different species between 5 and 6 million years ago.

Lucy was a what?
Lucy is the name for a set of fossil remains that have been dated back 3.2 million years ago. Lucy is thought to be a key in the link between modern humans and apes (well, other apes). From her bones, specifically the structure of her hips and knees, we can tell that Lucy could walk on two feet. Her brain was around 300-400 cubic centimetres (cc), which is similar in size to non-human primates like chimpanzees. Lucy was the first nearly-complete example of Australopithecus afarensis discovered, in 1974. She was named after the Beatles' song, "Lucy in the Sky with Diamonds". It was an important discovery because it showed for the first time that bipedalism evolved in humans before large brains did.

After Lucy, 2 million years ago, Homo habilis arrived on the scene. Tools found near the bones of Homo Habilis reveal a “handy man”, who could create simple tools for hunting. Homo habilis is thought to be around 5’ tall, and weigh about 45kg. From their teeth, it is believed that homo habilis had meat in their diet.

Homo erectus came next, living between 1.8 million and 300,00 years ago. It’s brain size is 900cc to 12000 cubic centimetres. From areas of the brain that had developed we believe that erectus had the power of speech. Plus, homo erectus had more sophisticated tools, weapons, fire and could cook his food.

Half a million years ago Homo sapiens (archaic) developed. Their brains averaged 1200cc, and had rounded skulls similar to the modern day humans. Then we arrived! It is estimated that modern humans developed 120,000 years ago. Our brains are around 13500cc.

You’re such a Neanderthal! (Or are you?)
No, you aren’t. Neanderthals lived in Europe and the Middle East between 150,000 and 35,000 years ago. Whilst there is debate, it is most commonly thought that Neanderthals coexisted with homo sapiens (archaic) but are a completely different species. They were tall creatures, around 5’6’’, with an extremely heavy skeleton, and probably stronger than modern man. They also had larger brains than modern man (1450 cc). It is unclear why Neanderthals died out. Some suggest they interbred with homo sapiens.

For more information on

Australopithecus (Great summary!)

http://www-geology.ucdavis.edu/~cowen/HistoryofLife/australopithecines.html

Neanderthals: http://www.bbc.co.uk/sn/tvradio/programmes/horizon/neanderthal_prog_summary.shtml


How Birds Evolved From Dinosaurs

Originally there are the large bipedal dinosaurs, like the T-rex and Allosaurus, and because of evolutionary pressures they begin to get smaller and smaller. Because of the decrease in body mass, they are having greater issues with body temperature control, because of an increase in body surface area in relation to total mass. Because of this, the light down that covered the larger dinosaurs became thicker, protecting them from the elements through a buffer zone.

When these dinosaurs got smaller, just a little larger than a pigeon, they were easier prey for the bigger dinosaurs, and so to escape from these predators, the little dinosaurs that were better equipped for tree climbing had a distinct advantage, and so moved into the redwood forests of the region which is now known as china.

Once they were in the trees, there was no-longer the predators that would be able to get them way up out of harms reach, and there is a new untapped food source, insects, which there is no competition with other species to get to. and so the only time that there is evolutionary pressures being pushed onto these animals is when they use up all the food in the tree that they are in. so the safest thing that they can do is jump from tree to tree.

So the down on the small dinosaurs evolves in its complexity into a feather like structure, which allowed the birds to glide from tree to tree. to make it so that they can glide further and further, they start to lighten up, so their bones hollow out, and become more fragile.

The mass extinction came.

The pterosaurs that were in the air, preventing these avian dinosaurs from leaving the forests of china, now due to environmental pressures are decreasing in number. This allows for Avian dinosaurs to leave the forests without being hunted, and so a beast plate re-enforces the chest, allowing for increase resistance for the wings to flap and so an increase in the distance that the birds could travel, and so allowing them to move out of the china and to increase in size.

The rest is history.

Episode 1: Science News

This week in the Petri Dish news:

A Sting In The Tale
Symbiosis is the process whereby two species evolve to live together in close and long-lasting interactions. Many people confuse symbiosis with mutualism, which is a type of symbiosis where the interaction is mutually beneficial, but parasitism (where one species benefits but the other one suffers, like tapeworms and their hosts) and commensualism (where one benefits but the other is unaffected, like vines living on trees) are also types of symbiosis.

You don't often get interactions more long lasting or intimate than incorporation of one species into another's genome, though. A group from the National Center for Scientific Research (CNRS) in Tours, France has found Braconid wasps have incorporated a Nudivirus into its genome. This isn't all that uncommon - it's the way retroviruses like HIV work. But unlike most cases, both the virus and the wasp have benefited. The virus is given somewhere warm and safe to live, and in return it gives the wasps the toxins they need to sting things.

The virus probably used to parasitise the wasps a few million years ago, and has since been fought to a standstill and come to benefit the wasp.

http://sciencenow.sciencemag.org/cgi/content/full2009/212/2

What's Not Hot in 2008 - The Climate

2008 was a cool year, temperature wise. Climatologists from NASA Goddard Institute for Space Studies (GISS) in New York City found that 2008 was the coldest year since 2000. But don’t get too excited because the report found that 2008 was still pretty damn hot. It was the ninth warmest year since records began in 1880. The cool climate was probably caused by a strong La Niña that was around in the first half of the year. La Niña and El Niño are opposite stages in the natural fluctuation in Pacific Ocean temperatures. La Niña is the cool phase. El Niño is the warmer phase, which typically follows within a year or two of its cooler cousin La Niña.

http://geology.com/nasa/nasa-maps-graphs-global-warming.shtml

Got Milk? Calcium Beats Cancer

Women with a higher intake of calcium have a lower risk of cancer. And both men and women with high calcium intakes have lower risks of colorectal cancer and other cancers of the digestive system. The findings are published in the February 23 issue of Archives of Internal Medicine. The research team analyzed data from 293,907 men and 198,903 women. The participants took a food frequency questionnaire between 1995 and 1996 reporting how much and how often they consumed dairy products and supplements. The results from that questionnaire were then linked with state cancer registries to identify new cases of cancer through 2003. Calcium intake was not associated with total cancer rate in men but was in women. According to the study to avoid an increased risk of cancer women should consuming 1,300 milligrams per day. One glass of milk gives around 300mg of calcium.

Yikyung Park (2009), Archives of Internal Medicine, 169(4):391-401.

http://archinte.ama-assn.org/cgi/content/abstract/169/4/391?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&fulltext=calcium+cancer&searchid=1&FIRSTINDEX=0&resourcetype=HWCIT